| 質粒名稱: | pSLIK CA Rac1 |
|---|---|
| 出品公司: | addgene |
| 目錄編號: | 84605 |
| 存儲實驗室: | Sanjay Kumar |
|
載體骨架基本信息 |
|
| 載體名稱: | pSLIK |
| 載體出品公司: | Iain Fraser (Addgene plasmid # 25734) |
| 載體類型: | 哺乳細胞表達 |
| 篩選標記: | sVenus |
| 載體抗性: | 氨芐霉素 |
| 生長溫度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷貝數: | 未知 |
|
插入基因信息 |
|
| 插入基因名稱: | Rac1 |
| 物種來源: | H. sapiens (human) |
| 基因突變: | RAC1 (a.k.a. MIG5, MRD48, Rac-1, TC-25, p21-Rac1) |
| 克隆方法: | 未知 |
| 5’測序引物 | pTYF-5 GTAGACATAATAGCAACAGAC |
| 3’測序引物 | hUBCpro-R CTAAGGCCGAGTCTTATGAGCAG |
|
文獻引用方法 |
|
| 材料和方法部分: | pSLIK CA Rac1 was a gift from Sanjay Kumar (Addgene plasmid # 84605 ; //n2t.net/addgene:84605 ; RRID:Addgene_84605) |
| 參考文獻部分: | Simultaneous and independent tuning of RhoA and Rac1 activity with orthogonally inducible promoters. MacKay JL, Kumar S. Integr Biol (Camb). 2014 Sep;6(9):885-94. doi: 10.1039/c4ib00099d. 10.1039/c4ib00099d PubMed 25044255 |
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